My specific area of research is to understand the role of ribosomes in bone marrow failure syndromes and cancer. Other areas of research include developing non-invasive and alternative DNA-based molecular tools for detection of specific mutations in cancer, developing transgenic zebrafish as cancer models and screening of natural antic-cancer compounds in zebrafish using HTS technology.
A plant biotechnologist by training, having 29 years of experience in academics and research. I have worked extensively on plant tissue culture, conservation, biodiversity and molecular markers in tree species and Pteridophytes. My team has screened several Pteridophytes for phytoremediation of heavy metals from contaminated soil and liquid systems. My current research interest is in vitro production of secondary metabolites of herbal medicinal plants.
Over the past 12 years I have worked on development of many new diagnostic techniques for a bacterial, viral pathogen as well as a parasite affecting humans using my expertise on LAMP technique and characterization (identification and function) of cytokines under different stress/infection. At present my research area and interest focusses on development of rapid molecular detection techniques, development of transgenic zebrafish lines for human diseases, Human /animal parasitology, and Molecular Immunology.
Doctor of Philosophy (Applied Biochemistry and Bioscience), University of Miyazaki, Japan, 2007
Master of Science (Aquaculture), West Bengal University of Animal Sciences, Kolkata, 2003
Molecular detection techniques
Japan-Asia Youth Exchange program in Science for undergraduates (SAKURA exchange program in science) coordinator, 2018
Japan-Asia Youth Exchange program in Science (SAKURA exchange program in science) awardee, 2016
Following Ph.D., Dr. Biswajit Maiti continued his research career at the College of Fisheries, Mangaluru. Later, he joined NUCSER as Assistant Professor in 2013. He has also worked as a visiting researcher in Food Microbiology at Obihiro University, Japan, during 2015 -2016. From the past decade, he has been working on the development of cutting-edge diagnostic methods for rapid detection microbial pathogens, deciphering outer membrane proteins based-antibiotic resistance of bacterial pathogens, and development of novel vaccines using biotechnological approaches. He has numbers of publications in scientific journals of repute, and book chapters to his credit, received research funds from various external agencies, and guiding students for their Ph.D., and postgraduate research dissertation.
Doctor of Philosophy (Bioscience), Mangalore University, 2012
Master of Science (Microbiology), Jiwaji University, 2006
Associate professor - Grade I, Nitte University Centre for science education and research, Mangaluru (2018- till date)
Assistant Professor, Nitte University Centre for science education and research, Mangaluru (2013- 2018)
Post-doctoral fellow (Food Microbiology), Obihiro University, Hokkaido, Japan (2015 - 2016)
Post-doctoral research associate, College of Fisheries, Mangaluru (2012 - 2013)
Development of diagnostic methods for the pathogenic bacteria.
Pathogenesis and antibiotic resistance mechanisms of bacteria.
Membrane proteins of Gram-negative bacteria.
Aquaculture and Marine Biotechnology.
Dr. (Mrs.) Gouri Ganguly Memorial Award for Young Scientist, Indian Science Congress, 2018.
Visiting researcher at Obihiro University, Japan, 2015
WFCC-MIRCEN-WDCM meeting on Microbial Resources Management and Utilization, IMCAS, Beijing, China, 2014
Young scientist award, Association of microbiologist of India, 2012
Short-term research fellowship, Nofima AS, Tromsø, Norway, 2010
TCDC Expert (laboratory management), FAO-UN, Dhaka, Bangladesh, 2010
Convener, Biotechnology in one health for pre-university college students sponsored by BRSI under the scheme Biotechnology popularization and skill development, 2019-2020.
Dr. Praveen Rai did PhD (Molecular Virology) from Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar. He joined NUCSER in 2014. He has 36 research publications in highly rated journals of international standard, mostly related to molecular characterization of microbial pathogens. Currently, his research focuses on molecular characterization of Dengue and Rotaviruses, development of point of care diagnostics for SARS-CoV-2, Dengue virus, Human Papillomavirus (HPV), Malaria Parasite and Leptospira, Screening of antiviral compounds, Host-Pathogen Interaction, RNA Interference and Phage based biocontrol technologies. His research work is supported by various agencies such as SERB, DST, DBT, ICMR and Nitte (DU).
Dr. Deekshit completed his PhD (Microbiology) from Manipal University and had been worked as a research associate at UNESCO MIRCEN for Marine Biotechnology (KVAFSU), Mangalore. He did his short-term post-doc at University College Dublin, Ireland in the year 2018. His research focus includes molecular characterization of nontyphoidal Salmonella with special consideration to their antibiotic resistance mechanism which led to the detection of class 1 integrons in Salmonella Weltevreden and silent antibiotic resistance genes in some non-typhoidal isolates of Salmonella. Further, he developed a PCR for the simultaneous detection of Salmonella Pathogenicity Island 2 and its antibiotic resistance genes. He has also developed a Miss Match mutation PCR assay for the detection of QRDR mutation in fluoroquinolone resistant E. coli. His research interest includes identifying the characteristics of multidrug resistant gut pathogens in simulated gut condition as well as determining the anticancer ability non-typhoidal Salmonella in vitro.
Dr. Krishna Kumar Ballamoole is a molecular microbiologist, obtained Ph.D. (Microbiology) from Manipal University. His research focuses on understanding and characterizing the genomic patterns that drives the evolution and infection capabilities of microbial pathogens of humans and animals. His work combines classical microbiological methods, molecular biology tool and next-generation sequencing strategies as fundamental tools to shed light on evolution, virulence capabilities and antibiotic resistance mechanisms in clinically relevant human and animal pathogens. In addition to pursuing the main research interest, the he is also involved in developing monoclonal antibody and nucleic acid based rapid diagnostics for the detection of pathogens.
Anurag Sharm is working as Assistant Professor at NUCSER, Nitte University. During his Ph.D. he studied the stress response of endosulfan on D. melanogaster, a non-target organism, at sub-organismal as well as organismal level. Further, his studies also highlight the potential of D. melanogaster as an alternative animal model for in-vivo assessment of environmental chemical mediated toxicity, which might have relevance to higher organisms. Later, he joined CNRS-Centre de Biologie du Développement, Toulouse, France as a postdoctoral researcher and worked on deciphering the function of the Drosophila hematopoietic niche to maintain blood cell homeostasis. Presently, he is engaged in the following areas-a) Development of an in vivo Drosophila model to study toxicant/radiation induced hematopoietic perturbations and b) Host-Microbe interaction and environmental toxicants.
Doctor of Philosophy (Cellular and Molecular toxicology), CSIR-IITR Lucknow and Jamia Hamdard, 2012
Master of Science (Biotechnology), Jiwaji University, 2005
Assistant Professor, Nitte Centre for science education and research, Mangaluru (2016 – till date)
Postdoctoral Fellow (Developmental Biology), Centre de Biologie du Développement (CBD), Toulouse, France (2012 - 2016)
Cellular and Molecular toxicology
Cellular immune response
Cellular and molecular stress response
DST-SERB Young scientist start-up grant award, 2017
Japan-Asia Youth Exchange program in Science (SAKURA exchange) awardee, 2018
Member, Lab Resource Committee, NUCSER
Member Secretary, Institutional committee against sexual harassment, NUCSER
Member, Institutional committee against substance abuse, NUCSER
D'Souza, L.C., Mishra, S., Chakraborty, A., Shekher, A., Sharma, A.,* Gupta, .SC.* (2020) Oxidative stress and cancer development: Are non-coding RNAs the missing links? Antioxidants & Redox Signaling [Epub ahead of print].
Sharma A, D'Souza C, Rai V, Gupta SC. (2019) Evaluation of Safety and Efficacy of Nutraceuticals Using Drosophila as an in Vivo Tool. In: Nutraceuticals in Veterinary Medicine. Gupta R, Srivastava A, Lall R. (eds). Springer, Cham. ISBN 978-3-030-04624- 8.
Louradour, I., Sharma, A., Morin-Poulard, I., Letourneau, M., Vincent, A., Vanzo, N., Crozatier, M. (2017). Reactive oxygen species-dependent Toll/NF-κB activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism. Elife 6:e25496. doi: 10.7554/eLife.25496.
Morin-Poulard, I., Sharma, A., Louradour, I., Vanzo, N., Vincent, A., Crozatier, M. (2016). Vascular control of the Drosophila hematopoietic niche: role of Slit/Robo signaling. Nature Communications 7:11634.
Sharma, A., Mishra, M., Shukla, A.K., Kumar, R., Abdin, M.Z., Chowdhuri, D.K. (2012). Organochlorine pesticide, endosulfan induced cellular and organismal response in Drosophila melanogaster. Journal of Hazardous Materials 221-222, 275-287.
Sharma, A., Mishra, M., Ravi Ram, K., Kumar, R., Abdin, M.Z., Chowdhuri, D.K. (2011). Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. Chemosphere 82: 370-376.
Sharma, A., Shukla, A.K., Mishra, M., Chowdhuri, D.K. (2011). Validation and application of Drosophila melanogaster as an in vivo model for the detection of double strand breaks by neutral Comet assay. Mutation Research/ Genetic Toxicology and Environmental Mutagenesis 721: 142-146.
All Publications: Google Scholar-
Decoding the molecular mechanism associated with ionizing radiation induced hematopoietic malignancy using Drosophila model system, funded by ICMR (Recommended), 2020-2022.
Deciphering the molecular mechanisms involved in hematopoietic perturbation induced by environmental chemical(s) using Drosophila as an animal model, funded by DST-SERB, 2017-2020 [Budget: 44.29 lakhs]
Elucidating the host-microbiome association in cadmium and/ nonyphenol exposed Drosophila melanogaster, funded by NitteDU, 2019-2021 [Bugget:1.9 lakhs]
Niche cues for stress hematopoiesis: insights from Drosophila, funded by NitteDU, 2019-2021 [3.0 lakhs]
With more than 15 years of experience in research and teaching, she has attended several conferences and been resource person at National and International level. Her latest visit to the U.S.A where she was invited as speaker to present a research paper on Zika virus at the First International Conference on Zika Virus 2017 held at Washington D.C, 2017. Her major research areas include Parasitology with special interest in Malaria in the city of Mangaluru where drug resistance is beginning to appear. She is presently working in the division of Bioinformatics and Computational genomics with increased surveillance and research on various viruses spreading across the globe.
Assistant Professor, Nitte University Center for Science Education and Research, Mangaluru (2013- till date)
Project Assistant, College of Fisheries, Mangaluru (2006-2013)
Molecular characterization of malarial plasmodium
Malaria and Antimicrobial Resistance
Sequencing and bioinformatics
Member, Board of Studies in Biological Sciences (UG and PG), Nitte DU
Member, Lab Vigilance Committee, NUCSER
Program Officer-NSS, NUCSER
Currently guiding Ph.D. student, working on the malaria problem in south-west India
Dr. Ramya Harsha is an Assistant Professor at NUCSER. She has obtained her Ph.D. degree from Department of Microbiology, University of Mysore in the year 2012. She joined NUCSER in 2013. Her research interests include virulence in bacterial pathogens, quorum sensing and isolation of anti-quorum sensing molecules from phytal sources and attenuation of quorum sensing in bacteria. She has secured second rank in MSc Microbiology from University of Mysore.
My research focuses on zoonotic pathogens, the use of antisense technologies to combat multi-drug resistant bacteria and the development of biocontrol strategies for bovine mastitis. My current research focuses on developing diagnostic assays for rabies virus.
All Publications: Google Scholar-
Conference publications- 7
'Klebsiella pneumoniae infections and antimicrobial drug resistance' chapter is published in the book entitled as Model organism for microbial pathogenesis, biofilm formation and antimicrobial discovery (2020), ISBN 978-981-15-1694-8.
Dr. Mamatha BS completed her Ph.D. from CFTRI, Mysore. Pursued teaching as my career and joined St. Aloysius College (Autonomous), Mangalore from 2014- 2017. Later, she joined NUCSER in 2017. She have 18 research articles and external reseach projects to her credits. Her research interest lies in two areas of food science, the bioavailability of lutein (xanthophyll carotenoid) in mice model and antihypertensive peptides from fish protein hydrolysates.
Doctor of Philosophy (Nutritional Biochemistry), Central Food Technological Research Center (CFTRI), University of Mysore, Mysore, 2013
Master of science (Food Science and Nutrition), University of Mysore, 2002
Nutrition Society of India (NSI) prize for the Best Oral Presentation during, 2019
Certificate of appreciation awarded in recognition of achievement/ dedicated and valuable service towards enhancing quality of education in "Sambhrama" 2015 and 2017, St. Aloysius college, Mangalore
Senior Research fellowship by the Indian Council of Medical Research (ICMR), 2007.
Awarded First prize for the poster presented, ICFOST-2005, NIMHANS, Bangalore.
Member, Hostel committee, NUCSER
Member, Academic committee, NUCSER
In-charge, Women cell, NUCSER
Dr. Sudarshan Kini received his Ph.D. degree in design and development of nanobiomaterials for anticancer drug delivery applications. Later he obtained his postdoctoral experience in Nanodiagnostics from Manipal Academy of Higher Education during 2015-2018. Presently he is working on the development of 3D animal cell culture and Nanosensors.
Best Paper award at International conference on Recent Advances in Materials Science and Biophysics at Mangalore, 2018.
Best poster in the International symposium on Nanotechnology and Cancer Theranostics (ISNACT-2015) at IIT Bombay, 2015
GATE qualified with 98.27 percentiles, All India rank 226, score 476.
Poojari, R., Sawant, A.V., Kini, S., Srivastava, R., Panda, D. (2020) Antihepatoma activity of multifunctional polymeric nanoparticles via inhibition of microtubules and tyrosine kinase. Nanomedicine (London, England) 15(4), 381-396.
Hegde, S., Anchan, B., Kamath, S. U., Kini, S., De, S., Patil, A. (2019) Design and development of ZnO based device for biosensing applications. In A. Asundi, & Q. Kemao (Eds.), Seventh International Conference on Optical and Photonic Engineering, icOPEN 2019 [112052A] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11205). SPIE. doi: 10.1117/12.2551081.
Kini, S., Kulkarni, S.D., Ganiga, V., Nagarakshit, T.K., Chidangil, S. (2019) Dual functionalized, stable and water dispersible CdTe quantum dots: Facile, one-pot aqueous synthesis, optical tuning and energy transfer applications. Materials Research Bulletin 110:57-66.
Kini, S., Ganiga, V., Kulkarni, S.D., Chidangil, S., George, S.D. (2018) Sensitive detection of mercury using the fluorescence resonance energy transfer between CdTe/CdS quantum dots and Rhodamine 6G. Journal of Nanoparticle Research 20:232.
Thakur, R., Kini, S., Kurkalang, S., Banerjee, A., Chatterjee, P., Chanda, A., Chatterjee, A., Panda, D., Mukherjee, A. K. (2016) Mechanism of apoptosis induction in human breast cancer MCF-7 cell by Ruviprase, a small peptide from Daboia russelii russelii venom. Chemico-biological interactions 258, 297-304.
Poojari, R., Kini, S., Srivastava, R., Panda, D. (2016) Intracellular interactions of electrostatically mediated layer-by-layer assembled polyelectrolytes based sorafenib nanoparticles in oral cancer cells. Colloids Surfaces B Biointerfaces 143:131-8.
All Publications: Google Scholar- https://scholar.google.com/citations?user=ixAqu2MAAAAJ&hl=en
A novel rapid sensitive fluorescent resonance energy transfer technique for selective detection and quantification of methicillin resistant Staphylococcus aureus, funded by DST-SERB-Early Career Research, 2019-2021 [Budget: 36.14 lakhs]
Nano-formulation of lutein to improve bioavailability in prevention of Age-Related Macular Degeneration, funded by ICMR, 2019-2021 [Budget: 24.28 lakhs]
In the current scenario where antibiotics no longer work, the need for alternative strategies have looked at phage therapy and I have been actively involved in research on phage-based therapeutics not only for human therapeutic use but also biocontrol in foods for more than 10 years.
Phage therapy and phage biology
I am presently working as a Senior Lecturer in NUCSER, have 6 years of teaching experience and am pursuing my Ph.D. in Biological Science stream. In addition, my expertise is in analysis of water quality parameters, microbiological techniques (soil and water), molecular techniques and toxicological studies (acute and chronic toxicity tests). I have been guiding the PG students as a guide and a Co-guide. I am also involved in analyzing external samples of food and water, and also involved in providing training to the personnel form different parts of the country. I have few GenBank submission in my name.
Secured Ph.D. in Biotechnology from CUSAT, and worked as Research Associate at IIT, New Delhi, Delhi University, Centre for Cellular & Molecular Biology, Hyderabad, and College of Fisheries, Mangalore. Received an International Fellowship for Short-term Postdoctoral work at Research Institutes for Fisheries & Aquaculture, Hungary. After, worked as Research Associate at Central Institute of Fisheries Education, Mumbai and NUCSER. Joined as Scientist (G-I) in 2019 at NUCSER.
Scientist - Grade I, Nitte University Center for Science Education and Research, Mangaluru (2019-till date)
Research Associate, NUCSER, Mangalore (2017-2019)
Research Associate, Central Institute of Fisheries Education, Mumbai (2015-2016)
Post-doctoral Fellow, Research Institutes for Fisheries & Aquaculture, HUNGARY (2014-2015)
Research Associate, College of Fisheries, Mangalore (2013-2014)
Research Associate, Centre for Cellular & Molecular Biology, Hyderabad (2009-2012)
Bioactive compounds; AMPs
Member, Students Welfare Committee, NUCSER
Sports Advisor, NUCSER
Member Secretary, Youth Red Cross, Nitte DU
Member, Lab Equipment Committee, NUCSER
After competing my Ph.D., I have worked as Research associate and joined NUCSER as Scientist (G-I) in 2019. My research interest includes Infectious Disease Biology (Bacteriology), Antimicrobial Resistance, Food Microbiology and Biopolymers.
The focus of my research is to understand the functioning of disordered and hybrid proteins, with respect to age associated diseases like neurodegeneration and cancer. A parallel interest is to develop amyloid based bio-nanocomposites for diverse applications. During my postdoc at Erasmus Medical Center, Rotterdam and PhD, I gained expertise in protein conformation studies using atomic force microscopy by single molecule imaging and analysis of DNA repair proteins (BRCA2-RAD51), study of polymorphism in self-assembled amyloids (α-synuclein) and functionalization of amyloids with gold nanoparticles (conductive nanowires). I joined NUCSER in 2020.
Doctor of Philosophy (Nanobiophysics), University of Twente, The Netherlands, 2016
Master of Science (Human Genomics), Panjab University, India, 2007
Best debater on "Impact of Nanotechnology on Society", 2014
Immunology foundation fellowship, 2009
EMBO travel award, 2009
UGC-NET lectureship, 2007
Gold medal Panjab University, 2007
Member: Student Welfare Committee, NUCSER
Coordinator, Departmental Journal Club and Work-Discussions, NUCSER
Arshdeep Sidhu, Małgorzata Grosbart, Humberto Sánchez, Bram Verhagen, Nick L L van der Zon, Dejan Ristić, Sarah E van Rossum-Fikkert, Claire Wyman (2020) Conformational flexibility and oligomerization of BRCA2 regions induced by RAD51 interaction. Nucleic Acids Research. (In press) https://doi.org/10.1093/nar/gkaa648 [IF: 11.501]
Sidhu, A*., Vaneyck, J., Blum, C., Segers-Nolten, I., Subramaniam, V. (2018) Polymorph-specific distribution of binding sites determines thioflavin-T fluorescence intensity in Î±-synuclein fibrils. Amyloid 25(3), 189-196.
Sidhu, A., Ristic, D., SÃ¡nchez, H., Wyman, C. (2018) The recombination mediator BRCA2: Architectural plasticity of recombination intermediates revealed by single-molecule imaging (SFM/TIRF). Methods in Enzymology 600: 347-374.
Sidhu, A., Segers-Nolten, I., Raussens, V., Claessens, M. M., Subramaniam, V. (2017). Distinct Mechanisms Determine Î±-Synuclein Fibril Morphology during Growth and Maturation. ACS chemical neuroscience 8(3), 538-547.
Sidhu, A., Segers-Nolten, I., Subramaniam, V. (2016). Conformational Compatibility Is Essential for Heterologous Aggregation of Î±-Synuclein. ACS chemical neuroscience 7(6), 719-727.
Raiss, C.C., Braun, T.S., Konings, I.B., Grabmayr, H., Hassink, G.C., Sidhu, A., le Feber, J., Bausch, A.R., Jansen, C., Subramaniam, V., Claessens, M.M. (2016). Functionally different Î±-synuclein inclusions yield insight into Parkinson's disease pathology. Scientific reports 6, 23116 .
Sidhu, A., Segers-Nolten, I., Subramaniam, V. (2014). Solution conditions define morphological homogeneity of Î±-synuclein fibrils. Biochimica et biophysica acta 1844(12), 2127-2134.
Sidhu, A., Surolia, A., Robertson, A. D., Sundd, M. (2011). A hydrogen bond regulates slow motions in ubiquitin by modulating a Î²-turn flip. Journal of molecular biology 411(5), 1037-1048.
Sidhu, A., Sundd, M. (2009) Protein biotechnology and its applications. Biotechnology applications, ed. C.S.K Mishra.; 213-230.
Conference publications- 01
Invited Talks- 05
Dr. Akshath completed his Ph.D. at CSIR-CFTRI, Mysore, with a specialization in Biosensors and Bio-imaging. His work was focused on nanoparticle-based biosensors to detect food contaminants and toxins using enzymes/aptamers as biorecognition elements. After his Ph.D., he was awarded the prestigious Marie Skłodowska-Curie fellowship (MCIFs) at the University of Leeds, UK. His work was focused on polyvalent glycan-nanoparticles to decipher HIV/Ebola infections using dendritic cell receptors-DCSIGN/R. His research at NUCSER is focused on developing nano-sensors to detect contaminants, bacteria, viruses etc. His research also focuses on glycan-nanoparticles to decipher bacterial/viral infections and inhibition. He has published papers in journals of international repute ex-Journal of American Chemical Society, Biosensors & Bioelectronics, etc.